The line-up announced by Jerzy Brzęczek on Monday includes two staple players who were previously left out from the squad and one who last played for the Polish national team in October 2015.
Winger Kamil Grosicki and defender Artur Jędrzejczyk are both returning to the squad after being previously left out by Brzęczek. Meanwhile, Paweł Olkowski, a right-back for English Championship club Bolton Wanderers, has been called up after a hiatus of three years.
Poland will on October 11 take on Portugal at home in the group stage of the UEFA Nations League, a new competitive football format in Europe.
Poland will play Italy at home on October 14.
Both matches will be played in the southern city of Chorzów.
Poland in early September drew 1-1 with Italy in their first match in the new UEFA Nations League. The match, at Stadio Renato Dall'Ara in Bologna, was a promising debut by Brzęczek as manager of the Polish national squad.
Brzęczek took over after the national squad's early exit from the World Cup in Russia.
Poland on September 11 drew 1-1 with Ireland in a friendly game after a lacklustre display that left fans disappointed.
Here is the full Poland squad for the October matches against Portugal and Italy:
Goalkeepers: Łukasz Fabiański (West Ham United), Łukasz Skorupski (Bologna FC), Wojciech Szczęsny (Juventus).
Defenders: Jan Bednarek (Southampton), Bartosz Bereszyński (Sampdoria), Kamil Glik (AS Monaco), Artur Jędrzejczyk (Legia Warsaw), Marcin Kamiński (Fortuna Duesseldorf), Tomasz Kędziora (Dynamo Kyiv), Paweł Olkowski (Bolton Wanderers), Rafał Pietrzak (Wisła Kraków), Arkadiusz Reca (Atalanta Bergamo).
Midfielders: Jakub Błaszczykowski (VfL Wolfsburg), Przemysław Frankowski (Jagiellonia Białystok), Jacek Góralski (Ludogorets Razgrad), Kamil Grosicki (Hull City), Damian Kądzior (Dinamo Zagreb), Mateusz Klich (Leeds United), Grzegorz Krychowiak (Lokomotiv Moscow), Rafał Kurzawa (Amiens SC), Karol Linetty (Sampdoria), Maciej Makuszewski (Lech Poznań), Damian Szymański (Wisła Płock), Piotr Zieliński (Napoli).
Forwards: Robert Lewandowski (Bayern Munich), Arkadiusz Milik (Napoli), Krzysztof Piątek (Genoa CFC).